Improvements in Human Keratinocytes and Antimicrobial Effect Mediated by Cell-Free Supernatants Derived from Probiotics

The skin acts as a physical and physiological barrier, thereby protecting the body from various environmental components stimuli. Cell-free supernatants (CFS) derived probiotics can improve functions retain moisture. In this study, to assess efficacy of CFS Ligilactobacillus salivarius Limosilactobacillus fermentum, we investigated barrier strengthening moisturizing effects in keratinocytes along with their antibacterial effects. We also determined adhesive on colorectal cells. To confirm improvements moisturization function mediated by keratinocytes, hyaluronic acid (HA) production, mRNA expression HA synthases (HAS)2, HAS3, FLG were measured. results showed that L. MG242 fermentum MG901 increased these genes production (2.40- 1.95-fold control). Additionally, inhibited growth S. aureus E. coli, demonstrating inhibitory against harmful pathogens observed skin. These indicate use may increase elimination potential pathogens.